Isotope labelling has been used for over 40 years to facilitate the study of protein structure using nuclear magnetic resonance (NMR) spectroscopy. During that time, traditional expression systems such as in vivo protein synthesis were the preferred options for fabricating the labelled proteins. However, nearly a decade ago, it was discovered that cell-free protein expression offers an abundance of beneficial properties making it more adapted to the addition of isotopes than first thought. Here, we give you our top five reasons to use a cell-free system to label your proteins for your NMR study…
Five reasons to label your proteins for NMR studies using cell-free systems.
Topics: Cell-free technology, Proteins
Mythbusting #10 : “Cell-free systems are only relevant for protein synthesis"
Cell-free systems are mainly used for in-vitro protein synthesis and can be considered as a powerful tool in genetic code reprogramming, involving the amber codon. In this use, the non-proteinogenic amino-acids are incorporated into proteins by charging them to suppressor-tRNAs molecules that reprogram the existing codons. However, cell-free systems are also used to engineer genetic circuits with applications for in-vitro biology or metabolic engineering.
Topics: Cell-free technology, Proteins, Biotech
Synthelis will attend the 3rd edition of the “Colloque POLEPHARMA Bioproduction”
Synthelis will attend the 3rd edition of the “Colloque POLEPHARMA Bioproduction” which takes place in Tours, December 20th.
Topics: Cell-free technology, Proteins
Validation of a new method to characterize proteoliposome in solution by Force Spectroscopy with Atomic Force Microscopy
In protein manufacturing, membrane proteins are well known to be challenging to produce in a functional form. SYNTHELIS’ patented cell-free technology allows customized expression and characterization of active proteins integrated in proteoliposomes. Membrane proteins are embedded directly into liposomes during the cell-free synthesis process allowing the proper folding of the protein. Following protein production, SYNTHELIS conducts quality controls to validate the target protein in terms of activity and structure.
Topics: Cell-free technology, Proteins
Applications for membrane proteins
Environment and definition of our activity
Topics: Cell-free technology, Proteins, Biotech
Synthelis : Our services
Topics: Cell-free technology, Proteins, Biotech