Five reasons to label your proteins for NMR studies using cell-free systems.

Posted by Bruno Tillier on 18-Sep-2019 11:19:00

Isotope labelling has been used for over 40 years to facilitate the study of protein structure using nuclear magnetic resonance (NMR) spectroscopy. During that time, traditional expression systems such as in vivo protein synthesis were the preferred options for fabricating the labelled proteins. However, nearly a decade ago, it was discovered that cell-free protein expression offers an abundance of beneficial properties making it more adapted to the addition of isotopes than first thought. Here, we give you our top five reasons to use a cell-free system to label your proteins for your NMR study…

Read More

Topics: Cell-free technology, Proteins

Mythbusting #10 : “Cell-free systems are only relevant for protein synthesis"

Posted by Bruno Tillier on 16-Jul-2019 09:46:00

Cell-free systems are mainly used for in-vitro protein synthesis and can be considered as a powerful tool in genetic code reprogramming, involving the amber codon. In this use, the non-proteinogenic amino-acids are incorporated into proteins by charging them to suppressor-tRNAs molecules that reprogram the existing codons. However, cell-free systems are also used to engineer genetic circuits with applications for in-vitro biology or metabolic engineering.

Read More

Topics: Cell-free technology, Proteins, Biotech

Synthelis will attend the 3rd edition of the “Colloque POLEPHARMA Bioproduction”

Posted by Nathalie Montémont on 17-Dec-2018 10:47:35

Synthelis will attend the 3rd edition of the “Colloque POLEPHARMA Bioproduction” which takes place in Tours, December 20th.

Read More

Topics: Cell-free technology, Proteins

Validation of a new method to characterize proteoliposome in solution by Force Spectroscopy with Atomic Force Microscopy

Posted by Bruno Tillier on 03-Dec-2018 11:08:11

In protein manufacturing, membrane proteins are well known to be challenging to produce in a functional form. SYNTHELIS’ patented cell-free technology allows customized expression and characterization of active proteins integrated in proteoliposomes. Membrane proteins are embedded directly into liposomes during the cell-free synthesis process allowing the proper folding of the protein. Following protein production, SYNTHELIS conducts quality controls to validate the target protein in terms of activity and structure.

Read More

Topics: Cell-free technology, Proteins

Applications for membrane proteins

Posted by Bruno Tillier on 27-Jul-2018 14:52:02
Read More

Topics: Proteins, Biotech

Environment and definition of our activity

Posted by Bruno Tillier on 09-Jul-2018 12:34:33

Read More

Topics: Cell-free technology, Proteins, Biotech

Synthelis : Our services

Posted by Bruno Tillier on 03-Jul-2018 12:06:26
Read More

Topics: Cell-free technology, Proteins, Biotech

Latest articles

Subscribe to our blog