Which extract is best for cell-free protein expression: wheat germ or E. coli?

Posted by Bruno Tillier on 05-Sep-2019 09:52:00

Cell-free protein expression offers a rapid, reliable technique for synthesis of proteins.  Successfully synthesizing large quantities of high-quality product requires finding the optimal protein expression conditions, such as the type of cellular extract used. Lysate choice for the cell- free reaction can affect expression feasibility, yield and cost. Which extract gives the best results? Here, we compare two of the most common: E. coli and wheat germ.

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Topics: Cell-free technology, Biotech

Mythbusting #10 : “Cell-free systems are only relevant for protein synthesis"

Posted by Bruno Tillier on 16-Jul-2019 09:46:00

Cell-free systems are mainly used for in-vitro protein synthesis and can be considered as a powerful tool in genetic code reprogramming, involving the amber codon. In this use, the non-proteinogenic amino-acids are incorporated into proteins by charging them to suppressor-tRNAs molecules that reprogram the existing codons. However, cell-free systems are also used to engineer genetic circuits with applications for in-vitro biology or metabolic engineering.

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Topics: Cell-free technology, Proteins, Biotech

Applications for membrane proteins

Posted by Bruno Tillier on 27-Jul-2018 14:52:02
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Topics: Proteins, Biotech

Environment and definition of our activity

Posted by Bruno Tillier on 09-Jul-2018 12:34:33

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Topics: Cell-free technology, Proteins, Biotech

Synthelis : Our services

Posted by Bruno Tillier on 03-Jul-2018 12:06:26
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Topics: Cell-free technology, Proteins, Biotech

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