Mythbusting #3 : "Cell-free systems are not suitable for large-scale industrial applications"

Posted by Bruno Tillier on 09-Apr-2019 13:30:00
Although traditional cell-free protein synthesis is only considered appropriate for small scale protein production, technological breakthroughs made since the turn of the century have drastically accelerated the development of this production platform, making it now suitable at the industrial scale. Previous limitations of the cell-free technology have been addressed: it is now scalable, consistent, robust and cost-effective (Zawada et al., 2011). Yields have been increased, the use of expensive reagents is no longer necessary, reactions lengthened, and reaction scales increased (Carlson et al., 2012), all of which contribute to making cell-free technology an interesting and efficient platform for industrial applications.
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Topics: Cell-free technology

Mythbusting #2: "Cell-free systems are slower than an E.coli cell-based system"

Posted by Bruno Tillier on 28-Mar-2019 08:00:00

cell free systems e.coli

The E. coli expression system is well-established, and its ability to produce large amounts of protein in a limited time is advantageous when compared to other expression systems, such as eukaryotic systems for which 3 or 4 weeks lead time are required.

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Topics: Cell-free technology

Mythbusting #1: "Cell-free systems are only relevant for membrane proteins"

Posted by Bruno Tillier on 28-Feb-2019 10:58:21

Cell free-systems are not only relevant for membrane proteins

Cell-free expression system offers advantages over cell-based expression systems for difficult-to-express proteins, not only for membrane proteins, but also for proteins with multiple disulfide bonds or cytotoxic proteins, for example.

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Topics: Cell-free technology

Synthelis will attend the 3rd edition of the “Colloque POLEPHARMA Bioproduction”

Posted by Nathalie Montémont on 17-Dec-2018 10:47:35

Synthelis will attend the 3rd edition of the “Colloque POLEPHARMA Bioproduction” which takes place in Tours, December 20th.

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Topics: Cell-free technology, Proteins

Validation of a new method to characterize proteoliposome in solution by Force Spectroscopy with Atomic Force Microscopy

Posted by Bruno Tillier on 03-Dec-2018 11:08:11

In protein manufacturing, membrane proteins are well known to be challenging to produce in a functional form. SYNTHELIS’ patented cell-free technology allows customized expression and characterization of active proteins integrated in proteoliposomes. Membrane proteins are embedded directly into liposomes during the cell-free synthesis process allowing the proper folding of the protein. Following protein production, SYNTHELIS conducts quality controls to validate the target protein in terms of activity and structure.

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Topics: Cell-free technology, Proteins

Applications for membrane proteins

Posted by Bruno Tillier on 27-Jul-2018 14:52:02
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Topics: Proteins, Biotech

Environment and definition of our activity

Posted by Bruno Tillier on 09-Jul-2018 12:34:33

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Topics: Cell-free technology, Proteins, Biotech

Synthelis : Our services

Posted by Bruno Tillier on 03-Jul-2018 12:06:26
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Topics: Cell-free technology, Proteins, Biotech

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