Mythbusting #9 : “Cell-free systems do not produce functional protein complexes"

Posted by Bruno Tillier on 09-Jul-2019 10:01:13

The open nature of cell-free systems allows the production of protein complexes much more easily than with in vivo systems.

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Topics: Cell-free technology

Mythbusting #8 : “Cell-free systems are complex; hence the increased uptake in recent years of cell-free kits.”

Posted by Bruno Tillier on 27-Jun-2019 13:54:49

The wide availability and convenience of cell-free kits has led to their increased use over the last few years.

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Topics: Cell-free technology

Mythbusting #7 : “Cell-free systems only work with plasmids”

Posted by Bruno Tillier on 13-Jun-2019 11:47:00

 

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Topics: Cell-free technology

Cell-free protein synthesis systems: the best chance for your project to succeed !

Posted by Bruno Tillier on 03-Jun-2019 14:08:31

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Topics: Cell-free technology

Mythbusting #6 : “Cell-free systems are not compatible with Good Manufacturing Practices (GMP grade).”

Posted by Bruno Tillier on 23-May-2019 11:16:00
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Topics: Cell-free technology

Mythbusting #5 : “Cell-free systems deliver low expression yields.”

Posted by Bruno Tillier on 09-May-2019 11:30:00

 

 
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Topics: Cell-free technology

Mythbusting #4 : “Cell-free systems only use bacterial extract.”

Posted by Bruno Tillier on 25-Apr-2019 14:03:00

Escherichia coli (E. Coli) is the most popular cell extract source for cell-free expression systems. However, extracts from wheat germ, rabbit reticulocytes or insect cells are also commonly used. Other mammalian cell lines are also more recently used for cell-free protein biosynthesis. Since they present different properties and advantages, the source of cell extract will be selected according to the properties of the protein to synthesize, such as the need for post-translational modifications, and other constraints, like yield, costs or convenience of cell extract preparation (Carlson et al., 2012; Casteleijn et al., 2013, Zemella et al., 2015).

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Topics: Cell-free technology

Mythbusting #3 : "Cell-free systems are not suitable for large-scale industrial applications"

Posted by Bruno Tillier on 09-Apr-2019 13:30:00
Although traditional cell-free protein synthesis is only considered appropriate for small scale protein production, technological breakthroughs made since the turn of the century have drastically accelerated the development of this production platform, making it now suitable at the industrial scale. Previous limitations of the cell-free technology have been addressed: it is now scalable, consistent, robust and cost-effective (Zawada et al., 2011). Yields have been increased, the use of expensive reagents is no longer necessary, reactions lengthened, and reaction scales increased (Carlson et al., 2012), all of which contribute to making cell-free technology an interesting and efficient platform for industrial applications.
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Topics: Cell-free technology

Mythbusting #2: "Cell-free systems are slower than an E.coli cell-based system"

Posted by Bruno Tillier on 28-Mar-2019 08:00:00

cell free systems e.coli

The E. coli expression system is well-established, and its ability to produce large amounts of protein in a limited time is advantageous when compared to other expression systems, such as eukaryotic systems for which 3 or 4 weeks lead time are required.

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Topics: Cell-free technology

Mythbusting #1: "Cell-free systems are only relevant for membrane proteins"

Posted by Bruno Tillier on 28-Feb-2019 10:58:21

Cell free-systems are not only relevant for membrane proteins

Cell-free expression system offers advantages over cell-based expression systems for difficult-to-express proteins, not only for membrane proteins, but also for proteins with multiple disulfide bonds or cytotoxic proteins, for example.

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Topics: Cell-free technology